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Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Welcome to Pakistan Research Repository Show full item record. Production and Purification of Triacylglycerol Hydrolase from Bacillus Specie, hec pakistan phd thesis education. Ahmed, Sana. Hydrolases are among industrially important enzymes which are produced globally and utilized to obtain various products.
As there is no shortage of lipid feed stocks the search of novel lipid hydrolases has recently attracted considerable attention. In particular, triacylglycerol hydrolases EC 3. Lipases have tremendous potential to catalyze esterification, transesterification and inter-esterification reactions besides hydrolyzing ester bonds of fats. They maintain catalytic ability in both aqueous and non-aqueous media andremain stable in organic solvents, hec pakistan phd thesis education.
Lipases are produced by living organisms but most copiously by microorganisms particularly by bacteria. Gram negative bacterial strains reportedly produce versatile lipases which are highly tolerant to organic solvents and therefore have greater industrial applications.
The present study was aimed to isolate a lipase producing bacterial strain from indigenous source followed by purification and characterization of lipase. In this context, a Gram negative lipolytic strain was isolated from kitchen grease of a commercial kitchen in Karachi.
The isolate SN-3 hec pakistan phd thesis education identified as Pseudomonas stutzeri through colonial, morphological and molecular techniques. The lipase productivity by SN-3 was boosted by manipulating various physical and chemical factors.
The highest lipase titer was accomplished at 40°C in an oil enriched medium pH Palm oil Purification of triacylglycerol hydrolase was initiated with removal of cells by centrifugation at xg at 4°C.
Ultrafiltration 30kDa cut off membrane was applied for concentration of cell free filtrate followed by lyophilization that led to purification by ~3. The SN-3 lipase was further purified by size exclusion chromatography using Sephadex G column 2.
The active fractions were pooled and concentrated. These steps stemmed to SDS-PAGE analysis xxiii revealed the molecular mass of lipase as 40kDa.
The single non-smearing band in native-PAGE was confirmed by zymography using alpha nephthyl acetate as substrate and fast red TR salt as a color developing agent. The kinetic properties of triacylglycerol hydrolase revealed that maximum lipid hydrolysis could occur in citrate buffer 50mM, pH6. Kinetically, triacylglycerol hydrolase from SN-3 appeared to prefer long chain saturated triacylglycerolsas and the maximum triacylglycerol hec pakistan phd thesis education Similarly, the enzyme appeared stable for up to 30 min to a broad pH range of 5.
Hec pakistan phd thesis education activity was obtained with isopropanol, as well. These results suggested that the triacylglycerol hydrolase is a highly solvent stable enzyme. Interestingly, some of the chemicals including metallic solution and detergents found to have stimulatory effect on triacylglycerol hydrolase activity by SN xxiv In brief, current research has established an effective addition in lipid hydrolytic enzymes validated through kinetic properties as well as tolerability of triacylglycerol hydrolase to various solvents and detergents.
Therefore, it is believed that the isolated enzyme has considerable potential as a potent catalyst for use in various industries. Sana Ahmad Biochemistry uok karachi.
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University of the Punjab Lahore Pakistan. She has done PhD in Education from the same institute. Her Area of specialization in PhD was curriculum. the Punjab, Lahore, Pakistan. 5. Managing Editor of a HEC recognized “Y” category Research Journal titled “Journal of Elementary Education” Publishes by Department of Elementary Education As a % legit paper writing website we guarantee to fulfill your task from Hec Pakistan Phd Thesis Education scratch within the next 24 hours. If you need, we could do it even faster. An essay can be Hec Pakistan Phd Thesis Education written in 1 hour, just say the word. Also, you'll be glad to know that more than 35% of orders are done before the deadline and delivered to you earlier than Hec Pakistan Phd Thesis Education at your service 24/7. Another great feature of our custom writing service is that we are available 24/7. There Hec Pakistan Phd Thesis Education is Hec Pakistan Phd Thesis Education a dedicated team of friendly customer support representatives who do their best to ensure that every customer has a pleasant customer experience
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